Human Thyrotrophin Receptor Monoclonal Antibody - Clone 4C1

Research tool for

  • Western blotting
  • Immunoprecipitation
  • Flow cytometry
  • Immunohistochemistry (frozen section)

 

Inhibits binding of TSH to TSH receptor by 70-80%.

 

The human thyrotrophin receptor (TSHR) is found primarily in epithelial cells of thyroid follicles where it induces (by cAMP) the phospholipase C and protein kinase A signal transduction pathways.  TSHR is the major pathogenic autoantigen in human autoimmune thyroid Graves’ disease.

 

Product Type

Hybridoma for monoclonal antibody


Immunogen

Bacterially expressed extracellular domain (residues 1-415 of humanTSHR fused to GST)

 

CrossReactivity

Cross-reacts with human and theoretically with rat, pig, sheep, mouse, dog, cat


Specificity

Detects residues 378 - 384 of human TSHR.

Recognises nativeprotein.


Raised in

Mouse


Isotype

IgG2a

             

           

References

  1. Shepherd PS, Da Costa CR, Cridland JC, Gilmore KS, Johnstone AP. 1999. Monoclonal antibodies that recognize thenative human thyrotrophin receptor. MolCell Endocrinol. 149: 197-206.
  2. Johnstone, AP, Cridland JC, Da Costa CR, Nussy SS, Shepherd. 2003. A functional site on the human thyrotrophinreceptor – potential therapeutic target in Graves’ disease. Clin Endocrinol. 59:437-441.
Patent Information:
For Information, Contact:
Melissa Rubock
IP & Licensing Manager
King's College London
melissa.rubock@kcl.ac.uk
Inventors:
Philip Shepherd
Alan Johnstone
Keywords: