SuperBUGS: Rabbit anti-MAP1B phospho (T1265)

Affinity purified anti-sera to the glycogen synthase kinase (GSK3b) mediated nonprimed phosphorylation site of Microtubule Associated Protein 1B (MAP1B). MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones.

 

King’s Developer

Professor Phillip R. Gordon-Weeks


Reagent name

SuperBUGS: Rabbit anti-MAP1B phospho (T1265)


Immunogen (peptide sequence + location; recombinant protein; native protein from which species)

CSPIEKTPLGER, in which threonine was phosphorylated, were synthesised and conjugated to keyhole limpet haemocyanin


Epitope

SPIEKTPLGER in mouse MAP1B


Positive control (e.g. tissue/cell type or cell line)

Embryonic neurons, rodent neonatal brain extract


Raised in

Rabbit


Amount available if polyclonal

(Affinity purified) 4 ml


Cross reactivity

Mammals, chick, snake, frog, dogfish


Specificity

Phospho-specific


Tested Applications with suggested dilutions in specified applications

Immunoblotting (1:3,000), Immunostaining (tissue sections 1:900); Immunofluorescence (cell culture 1:200)

 

References

  1. Trivedi et al., 2005, J. Cell Sci. 118, 993-1005
  2. Scales et al., 2009,  J. Cell Sci. 122, 2424-2435

 

 

Patent Information:
For Information, Contact:
Salma Ishaq
IP & Licensing Manager
King's College London
salma.ishaq@kcl.ac.uk
Inventors:
Phillip Gordon-Weeks
Keywords: