HIV-1 p24 Gag monoclonal antibody - Clone 24-3

Reagent Name

HIV-1 p24 Gag monoclonal antibody (#24-3)

Description

Hybridoma expressing antibodies to human immunodeficiency virus type 1

Immunogen

Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 Gag protein (clade B, HXB-3 isolate)

Relevance

p24 is encoded by the HIV gag gene and is an important structural component of the capsid core of the HIV particle

Cross reactivity

Not tested

Specificity

Epitope not mapped

Tested applications

Western blots for clade B isolates of HIV; immunofluorescence assays.  (not useful for immunoprecipitation assays)

Raised in

Mouse

Clonality

Monoclonal

Isotype

IgG_{1, k}

References

1. Simon JH, Fouchier RA, Southerling TE, Guerra CB, Grant CK, Malim MH. The Vif and Gag proteins of human immunodeficiency virus type 1 colocalize in infected human T cells. Journal of Virology 71:5259-67, 1997.
2. Fouchier RA, Meyer BE, Simon JH, Fischer U, Malim MH. HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import. EMBO J 16:4531-9, 1997.
3. Simon JH, Carpenter EA, Fouchier RA, Malim MH. Vif and the p55(Gag) polyprotein of human immunodeficiency virus type 1 are present in colocalizing membrane–free cytoplasmic complexes. Journal of Virology 73:2667-2674, 1999.