HIV-1 p24 Gag monoclonal antibody - Clone 24-4

Reagent Name

HIV-1 p24 Gag monoclonal antibody (#24-4)

Description

Hybridoma expressing antibodies to human immunodeficiency virus type 1

Immunogen

Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 Gag protein (clade B, HXB-3 isolate)

Relevance

p24 is encoded by the HIV gag gene and is an important structural component of the capsid core of the HIV particle

Cross reactivity

Cross-reactivity with a ~65 kDa species in western blot

Specificity

Epitope not yet mapped

Tested applications

Western blots for clade B isolates of HIV; immunofluorescence/FACS assays (paraformaldehyde-fixed samples); immunoprecipitation assays

Raised in

Mouse

Clonality

Monoclonal

Isotype

IgG_{2b, k}

References

1. Simon JH, Fouchier RA, Southerling TE, Guerra CB, Grant CK, Malim MH. The Vif and Gag proteins of human immunodeficiency virus type 1 colocalize in infected human T cells. Journal of Virology 71:5259-5267, 1997.
2. Fouchier RA, Meyer BE, Simon JH, Fischer U, Malim MH. HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import. EMBO J 16:4531-4539, 1997.