HIV-1 integrase (IN) monoclonal antibody - Clone IN-2

Reagent name

HIV-1 integrase (IN) monoclonal antibody (#IN-2)

Description

Hybridoma expressing antibodies to human immunodeficiency virus type 1

Immunogen

Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 integrase protein (clade B, HXB-3 isolate)

Relevance

HIV-1 expresses the integrase (IN) protein as part of the polymerase gene.  It is the viral enzyme that catalyses the insertion of viral DNA into the chromosomal DNA of an infected cells.  Integrase is essential for viral growth and is a therapeutic target.

Cross-reactivity

No cross-reactivity with non-HIV-1 integrase proteins has been observed

Specificity

Epitope not yet mapped

Tested applications

Western blots; immunofluorescence assays; immunoprecipitation assays

Raised in

Mouse

Clonality

Monoclonal

Isotype

IgG_{1, k}

References

1. Bouyac-Bertoia M, Dvorin JD, Fouchier RAM, Jenkins Y, Meyer BE, Wu LI, Emerman M, Malim MH. HIV-1 infection requires a functional integrase NLS. Molecular Cell 7:1025-1035, 2001.
2. Gaddis NC, Chertova E, Sheehy AM, Henderson LE, Malim MH. Comprehensive investigation of the molecular defect in vif-deficient human immunodeficiency virus type 1 virions. Journal of Virology 77:5810-5820, 2003.